Chromaffin Cells
English | 2023 | ISBN: 1071626701 | 367 Pages | PDF (True) | 13 MB
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Therapeutic Applications of Mesenchymal Stem Cells in Veterinary Medicine
English | 2022 | ISBN: 981193276X | 732 Pages | PDF EPUB (True) | 13.4 MB
This book reviews the potential therapeutic and reproductive applications of mesenchymal stem cells in veterinary regenerative and reproductive medicine. The systemic approach focuses on musculoskeletal structures like cartilage, bone, muscle, tendon, ligaments and nervous tissues. It also focuses on other body systems like gastrointestinal, cardiovascular, urogenital, respiratory and integumentary system. Besides, the special glands or organs like endocrine glands and eye and its adnexa are also focused. The book chapters discusses the problems and the need for regenerative medicine employing MSCs. It provides an ex vivo basis of MSCs therapeutics and reproductive potential followed by their in vivo applications. The book further provides an understanding on the behavior and mechanisms of action of mesenchymal stem cells. The book also abridges challenges and provides future prospects of mesenchymal stem cells in clinical and reproductive applications. As such, the book offers a valuable resource for students, veterinarians, and scientists working in the regenerative and reproductive sciences in human and veterinary medicine.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition By R. Ian Freshney(auth.)
2010 | 772 Pages | ISBN: 0470528125 | PDF | 87 MB
This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs.PowerPoint slides of the figures as well as other supplementary materials are available at a companion website:www.wiley.com/go/freshney/cellcultureContent: Chapter 1 Introduction (pages 1-10): Chapter 2 Biology of Cultured Cells (pages 11-23): Chapter 3 Laboratory Design, Layout, and Equipment (pages 25-36): Chapter 4 Equipment and Materials (pages 37-56): Chapter 5 Aseptic Technique (pages 57-70): Chapter 6 Safety, Bioethics, and Validation (pages 71-88): Chapter 7 Culture Vessels and Substrates (pages 89-98): Chapter 8 Defined Media and Supplements (pages 99-114): Chapter 9 Serum?Free Media (pages 115-132): Chapter 10 Preparation and Sterilization (pages 133-162): Chapter 11 Primary Culture (pages 163-186): Chapter 12 Subculture and Cell Lines (pages 187-206): Chapter 13 Cloning and Selection (pages 207-225): Chapter 14 Cell Separation (pages 227-237): Chapter 15 Characterization (pages 239-268): Chapter 16 Differentiation (pages 269-278): Chapter 17 Transformation and Immortalization (pages 279-297): Chapter 18 Contamination (pages 299-315): Chapter 19 Cryopreservation (pages 317-334): Chapter 20 Quantitation (pages 335-364): Chapter 21 Cytotoxicity (pages 365-381): Chapter 22 Specialized Cells (pages 383-432): Chapter 23 Stem Cells, Germ Cells, and Amniocytes (pages 433-462): Chapter 24 Culture of Tumor Cells (pages 463-479): Chapter 25 Three?Dimensional Culture (pages 481-495): Chapter 26 Scale?Up and Automation (pages 497-515): Chapter 27 Specialized Techniques (pages 517-532): Chapter 28 Training Programs (pages 533-568): Chapter 29 Problem Solving (pages 569-591): Chapter 30 In Conclusion (page 593):

Dye F Dictionary of Stem Cells, Regenerative Medicine, 2017 | 17.47 MB
N/A | 379 Pages

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English | 2022 | ISBN: 3110344203 | 530 pages | True PDF EPUB | 25.49 MB
The operation of everything in the universe needs a special „material"-energy. The earth is no exception. There are many kinds of energy sources on earth. But where does the earth's energy come from? The answer is that everything grows under the sun. Developing renewable energy is of strategic importance to achieve sustainable energy supply. Simulating natural photosynthesis is the ultimate goal of effi cient solar energy conversion. Photovoltaic technology has been widely used in industry and will be one of the major energy sources in the future. Developing new materials and structures, the photoelectric conversion effi ciency of solar cells will be improved day by day, and solar cells will attract more and more attention. This book presents principles of solar photovoltaic conversion, and introduces the physical and chemical processes involved. Mechanisms which affect solar cell performance are also discussed.
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Cognitive Evolution: From Single Cells to the Human Mind
English | 2022 | ISBN: 0367679558 | 397 Pages | PDF (True) | 29 MB
Cognitive Evolution provides an in-depth exploration of the natural history of cognition, from the beginning of life on Earth to present-day humans. Drawing together evolutionary, comparative, and neuroscience research, the book brings a unique cognitive perspective to evolutionary psychology.

Songyuan D Dye-sensitized Solar Cells 2022 | 12.36 MB
N/A | 530 Pages

Title: EltaMD UV Clear SPF 46 Tinted Face Sunscreen, Broad Spectrum Sunscreen for Sensitive Skin and Acne-Prone Skin, Oil-Free Mineral-Based Sunscreen, Sheer Face Sunscreen with Zinc Oxide, 1.7 oz Pump
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Srinivas S. Somanchi, "Natural Killer Cells: Methods and Protocols"
English | 2016 | pages: 383 | ISBN: 1493936824 | PDF | 12,7 mb
This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such asex vivoexpansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors usingretroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis.Written in the highly successfulMethods in Molecular Biologyseries format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Induced Pluripotent Stem Cells and Human Disease: Methods and Protocols by Kursad Turksen
English | PDF | 2022 | 426 Pages | ISBN : 1071625845 | 14.6 MB
This detailed volume presents a series of protocols that are representative of recent developments and improvements in induced pluripotent stem cells (iPS cells) and corresponding human disease models. Reflecting the latest technology for generating induced pluripotent stem cells (iPS cells) and their initial characterization, the book explores techniques invaluable both for studies of disease-specific cell types and for their potential applications in regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.

Stem Cells: Current Challenges and New Directions By Maximilien Bencze, Ingo Riederer (auth.), Kursad Turksen (eds.)
2013 | 373 Pages | ISBN: 1461480655 | PDF | 6 MB
This volume looks at the state-of-the-science in stem cells, discusses the current challenges, and examines the new directions the field is taking. Dr. Turksen, editor-in-chief of the journal "Stem Cell Reviews and Reports," has assembled a volume of internationally-known scientists who cover topics that are both clinically and research-oriented. The contents range from sources of stem cells through their physiological role in health and disease, therapeutic applications in regenerative medicine, and ethics and society. An initial overview and a final summary bookend the contents into a cohesive and invaluable volume.