Cell Biology and Translational Medicine, Volume 16:
Stem Cells in Tissue Regeneration, Therapy and Drug Discovery
English | 2022 | ISBN: 3031106377 | 479 Pages | PDF EPUB (True) | 17 MB

Hillman H Evidence Based Cell Biology Clinical Research 2008 | 24.88 MB
N/A | 609 Pages

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Elodie Segura, Nobuyuki Onai, "Dendritic Cell Protocols"
English | 2016 | pages: 335 | ISBN: 1493936042 | PDF | 12,6 mb
The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Hematopoietic Stem Cell Protocols By Elaine Dzierzak, Marella de Bruijn (auth.), Christopher A. Klug PhD, Craig T. Jordan PhD (eds.)
2002 | 333 Pages | ISBN: 0896038122 | PDF | 3 MB
Hematopoietic stem cells have an enormous clinical importance for bone marrow transplantation, hematopoietic gene therapy, solid organ transplantation, and somatic tissue regeneration. In Hematopoietic Stem Cell Protocols, a group of leading stem-cell investigators describe in detail the key laboratory methods for investigating this critically important cell type. Varied in scope, and richly detailed with step-by-step instructions and notes on the pitfalls to be avoided, these readily reproducible protocols apply hematopoietic stem cells isolated from mouse and human tissues of both embryonic and adult origin. The many approaches described employ not only molecular and cell biology techniques, but also animal model systems, and include hematopoietic stem cell expansion protocols and methods for the purification and genetic modification of stem cells. Many of the protocols are contributed by those researchers who first developed and introduced the procedures to the scientific community. Comprehensive and state-of-the-art, Hematopoietic Stem Cell Protocols offers both novice and experienced investigators alike an invaluable compendium of highly practical techniques for studying a wide variety of stem cell topics, including blood formation, blood-based disease, and such blood cancers as leukemia.

Epithelial Cell Culture Protocols By Nobuhiro Ibaraki (auth.), Clare Wise (eds.)
2002 | 407 Pages | ISBN: 0896038939 | PDF | 4 MB
Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. These readily reproducible techniques describe all the commonly used protocols, as well as those that are more specialized, including several aimed at clinical investigation. The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders. Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.

Cell Neurobiology Techniques By Alan A. Boulton, Glen B. Baker, Alan N. Bateson (auth.)
1999 | 391 Pages | ISBN: 0896035107 | PDF | 15 MB
Cell Neurobiology Techniques is the second work updating and expanding the best-selling inaugural volume of Humana Press's warmly received Neuromethods series, General Neurochemical Techniques (vol. 1). The cutting-edge techniques detailed in this new edition include those that are particularly popular in multidisciplinary neuroscience research. There are readily reproducible methods for establishing neural cell cultures, measuring enzymes and their inhibitors, and using quantitative autoradiography to study monoamine uptake sites and receptors in the brain. Additional methods cover the use of flow cytometry to study developmental neurobiology, applications of magnetic resonance spectroscopy (MRS) to human brain metabolism, and the study of drug metabolism. The companion volumes, In Vivo Neuromethods and In Vitro Neurochemical Techniques, review both in vivo methods and in vitro neurochemical and molecular neurobiological approaches. Like the original, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.

Neuronal Cell Culture: Methods and Protocols By Jennifer Gordon, Shohreh Amini, Martyn K. White (auth.), Shohreh Amini, Martyn K. White (eds.)
2013 | 165 Pages | ISBN: 1627036393 | PDF | 5 MB
In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved. Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.

hongwei Chen, Jean-Pol Dodelet, Jiujun Zhang, "Non-Noble Metal Fuel Cell Catalysts"
English | 2014 | pages: 448 | ISBN: 3527664912, 352733324X | PDF | 119,1 mb
Written and edited by top fuel cell catalyst scientists and engineers from both industry and academia, this is the first book to provide a complete overview of this hot topic. It covers the synthesis, characterization, activity validation and modeling of different non-noble metal electrocatalysts, as well as their integration into fuel cells and their performance validation, while also discussing those factors that will drive fuel cell commercialization.

Handbook of Cell Biosensors by Gérald Thouand
English | EPUB | 2022 | 997 Pages | ISBN : 3030232166 | 116.9 MB
This handbook is an interdisciplinary and comprehensive reference covering all aspects of cell biosensors. It is divided into four main sections which are led and organized by numerous international experts. The scope of coverage includes:

Engineering Materials for Stem Cell Regeneration by Faheem A. Sheikh
English | PDF,EPUB | 2021 | 709 Pages | ISBN : 9811644195 | 97.2 MB
This book reviews the interface of stem cell biology and biomaterials for regenerative medicine. It presents the applications of biomaterials to support stem cell growth and regeneration. The book discusses the stem cell interactions' with nanofiber, gradient biomaterial, polymer- and ceramic biomaterials, integrating top-down and bottom-up approaches, adhesive properties of stem cells on materials, cell-laden hydrogels, micro-and nanospheres, de-cellularization techniques, and use of porous scaffolds. Further, this book provides a basic introduction to the fabrication techniques for creating various biomaterials that can be used for stem cell differentiation.